Avoiding accuracy-limiting pitfalls in the study of protein-ligand interactions with isothermal titration calorimetry

Sarah E. Boyce, Joel Tellinghuisen, and John D. Chodera.
Manuscript prior to submission. [bioRxiv] [PDF]
Supplementary files: ITC worksheet [PDF] [XLSX] [ODS]

We show how to avoid common accuracy-limiting mistakes in isothermal titration calorimetry, and provide a simple spreadsheet to aid in propagating the dominant source of uncertainty (titrant concentration errors) into the resulting thermodynamic parameters.

Keywords: isothermal titration calorimetry; ITC; propagation of error; entropy-enthalpy compensation