Our laboratory is equipped with a variety of computational and experimental resources for the quantitative study of protein-ligand association.
960 hyperthreaded cores - each node provides 32 hyperthreads with 8 GB/hyperthread
Torque/Moab batch queue scheduler and resource manager - provides advanced scheduling and reservation of CPU, GPU, memory, and license resources
Laboratory automation system overview
Thermo Fisher Momentum software - facilitates scheduling of multiple experiments simultaneously
Thermo BenchTrak Orbitor - automated 360-degree plate manipulator with internal regrip station, mounted on a 3.6m linear track. Used to integrate various other pieces of wet lab equipment into a single automation system.
Bionex HiG4 robotic centrifuge - microplate centrifugation up to 5000g
4x Inheco Incubator-Shaker DWP - bacterial culture and incubation in 96-well deepwell plates
Agilent PlateLoc - automated plate sealer
Agilent VCode - automated barcode printing and application for microplates
Thermo Cytomat Hotel - for random access to hundreds of microplates
Thermo MultiDrop Combi reagent dispenser - for rapid filling of microplates with common reagents
Tecan HP D300 - direct digital titration of compounds in DMSO
Tecan Infinite M1000PRO multifunction plate reader - equipped for absorption, fluorescence, fluorescence polarization, luminescence, and alphascreen assays, with injectors for kinetic assays
Caliper LabChip GX II - microfluidic quantitation and size analysis of proteins and nucleic acids
Roche LightCycler 480 - qPCR for standard PCR and thermofluor protein denaturation melts
Tecan EVO 200 liquid-handling workstation
Liquid Handling Arm (LiHa) - liquid-based syringe-actuated pipetting arm for dispensing volumes up to 1000 uL without the need for disposable pipette tips
Air LiHa - air based pipetting arm with disposable tips for accurate dispensing of 1-200 uL volumes
Tecan Te-VacS - vacuum station with two independently controlled vacuum manifolds
4x Torrey Pines EchoTherm RIC20 Peltier units - for heating and cooling
Compound stock solution preparation Compound storage
LabMinds Revo automated buffer maker - fully automated, reproducible, traceable preparation of buffers in liter quantities, requested by built-in tablet or on-the-go from your mobile device
Roylan Developments Ltd StoragePods - multichannel compound storage unit to prevent moisture absorption of samples. Storage capacity of 252 shallow well plates or 72 tube racks. [controller] [storage units]
Mettler-Toledo Quantos automatic gravimetric solution preparation system - powder and liquid dosing heads enable accurate preparation of compound solution stocks.
Integrated Mettler-Toledo QD206DR balance has 0.005mg @ 81g / 0.01mg @ 220g readability.
Four-tiered Bio-Rad NGC Quest 10 Plus Chromatography System - for column chromatography. Equipped with 10 mL/min pumps, multi-wavelength (UV/Vis) and conductivity detectors, pH sensing, automated sample injection, buffer blending valve, with the capacity to run multiple columns in succession.
Malvern MicroCal Peaq-ITC Automated for automated thermodynamic measurements of biomolecular interactions with the capacity to run up to four 96-well plates per experiment.
Mettler-Toledo DM40 density meter with 0.0001 g/cm3 accuracy, 0-3 g/cm3 range, and temperature measurement range of 0-91 C using 1 mL sample volumes.
Banner image by Christina Johnson