An open library of human kinase domain constructs for automated bacterial expression

Daniel L. Parton, Sonya M. Hanson, Lucelenie Rodríguez-Laureano, Steven K. Albanese, Scott Gradia, Chris Jeans, Markus Seeliger, and John D. Chodera.
Manuscript prior to publication: [bioRxiv]
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Human kinase catalytic domains---the therapeutic target of selective kinase inhibitors used in the treatment of cancer and other diseases---are notoriously difficult and expensive to express in insect or human cells. Here, we utilize the phosphatase co-expression technology developed by Markus Seeliger (now at Stony Brook) to develop a library of human kinase catalytic domains for facile and inexpensive expression in bacteria.